Determination of adhesion force between single cell pairs generated by activated GpIIb-IIIa receptors

Blood. 1993 Jan 15;81(2):419-23.

Abstract

A biophysical approach was used to directly determine the avidity of the junction between two Chinese hamster ovary (CHO) cells bearing recombinant GpIIb-IIIa in the presence and absence of fibrinogen. Micromanipulation was used to induce conjugation of the cell pairs with or without activating the GpIIb-IIIa molecules with monoclonal antibody (MoAb) 62. Activation of GpIIb-IIIa caused an increase in the force required to separate the conjugates. The molecular bonding force between cells bearing activated GpIIb-IIIa and fibrinogen molecules was found to be 2.1 x 10(-7) dyne, which is 3.7 times higher than that between nonactivated GpIIb-IIIa and fibrinogen (5.7 x 10(-8) dyne). The results provide a quantitative assessment of the molecular bonding force between fibrinogen and the GpIIb-IIIa expressed on cell surface. The findings indicate that the activation of GpIIb-IIIa leads to an increase in the adhesive force in CHO cell aggregation by increasing the strength of the GpIIb-IIIa-fibrinogen bonds rather than the number of these bonds.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • CHO Cells
  • Cell Adhesion* / drug effects
  • Cloning, Molecular
  • Cricetinae
  • Fibrinogen / metabolism*
  • Fibrinogen / pharmacology
  • Gene Library
  • Humans
  • Kinetics
  • Leukemia, Erythroblastic, Acute
  • Macromolecular Substances
  • Platelet Membrane Glycoproteins / genetics
  • Platelet Membrane Glycoproteins / immunology
  • Platelet Membrane Glycoproteins / physiology*
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism
  • Time Factors
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Macromolecular Substances
  • Platelet Membrane Glycoproteins
  • Recombinant Proteins
  • Fibrinogen