Antibodies to recombinant fragment 212-276 of protein C specifically recognize the intact human molecule

Biochim Biophys Acta. 1993 Feb 13;1161(2-3):187-93. doi: 10.1016/0167-4838(93)90212-a.

Abstract

The peptide fragment Pro212-Ile276 of human protein C was produced as a part of a fusion protein in Escherichia coli. The identity of the peptide was confirmed by immunoblotting experiments using specific antibodies to intact protein C. The peptide Pro212-Ile276 was isolated from the fusion protein after mild hydrolysis with formic acid by gel filtration and reverse-phase HPLC. This peptide fragment was used to produce antibodies specific for the heavy chain of protein C which recognized native protein C present in blood plasma. Antibodies to intact protein C reacted also with the Pro212-Ile276 peptide fragment, indicating that this region is immunogenic in intact protein C and may represent a native epitope.

MeSH terms

  • Amino Acid Sequence
  • Antibody Specificity
  • Base Sequence
  • Binding, Competitive
  • Blotting, Western
  • Chromatography, High Pressure Liquid
  • DNA, Recombinant
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli
  • Humans
  • Molecular Sequence Data
  • Peptide Fragments / immunology*
  • Peptide Fragments / metabolism
  • Protein C / immunology*
  • Protein C / metabolism
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism

Substances

  • DNA, Recombinant
  • Peptide Fragments
  • Protein C
  • Recombinant Proteins