Abstract
Studies have been performed to investigate the regulation of arginine vasopressin (AVP) mRNA expression in fetal hypothalamic cultures. AVP mRNA-positive neurones were identified by in-situ hybridization histochemistry, and changes in mRNA expression were quantitated by nuclease protection assay. Both protein kinase C and protein kinase A activators increased the expression of AVP mRNA, in contrast to dexamethasone, which inhibited the responses to both protein kinase C and protein kinase A activation.
MeSH terms
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Animals
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Arginine Vasopressin / genetics*
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Cells, Cultured
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Colforsin / pharmacology
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Dexamethasone / pharmacology
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Down-Regulation
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Fetus / metabolism
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Gene Expression Regulation / drug effects
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Hypothalamus / drug effects
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Hypothalamus / metabolism*
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In Situ Hybridization
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Protein Kinase C / metabolism
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Protein Kinases / metabolism
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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Rats
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Tetradecanoylphorbol Acetate / pharmacology
Substances
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RNA, Messenger
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Arginine Vasopressin
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Colforsin
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Dexamethasone
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Protein Kinases
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Protein Kinase C
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Tetradecanoylphorbol Acetate