Lymphokine production by mesenteric lymph node cells from BALB/C mice during Hymenolepis nana infection

Int J Parasitol. 1993 Feb;23(1):51-6. doi: 10.1016/0020-7519(93)90097-i.

Abstract

Mesenteric lymph node cells (MLNC) prepared from BALB/c mice during infection with Hymenolepis nana proliferated extensively when cultured in the presence of soluble egg antigen, as assessed by measuring 3H-thymidine incorporation. Analysis of Hymenolepis-specific proliferative cells in MLNC by using monoclonal antibody specific for mouse T lymphocyte surface antigens revealed that the proliferative response of MLNC was mediated by Thy-1.2+, L3T4+ cells, that is, helper T cells. Supernatant of MLNC cultured with egg antigen contained large amounts of interleukin-2 and interferon-gamma, but only low levels of interleukin-5. The titer of these cytokines did not correlate with the interval between oral infection and collection of MLNC. These results strongly indicate that the Th1 subtype of helper T lymphocytes respond well to stimulation of H. nana egg antigen and suggest that acute inflammatory responses are involved in host-protective immunity to H. nana.

MeSH terms

  • Animals
  • Cells, Cultured
  • Hymenolepiasis / immunology*
  • Interferon-gamma / metabolism
  • Interleukin-2 / metabolism
  • Lymph Nodes / immunology*
  • Lymphocyte Activation
  • Lymphokines / metabolism*
  • Male
  • Mesentery / immunology*
  • Mice
  • Mice, Inbred BALB C
  • T-Lymphocytes, Helper-Inducer / immunology*

Substances

  • Interleukin-2
  • Lymphokines
  • Interferon-gamma