Estrogen-inducible and liver-specific expression of the chicken Very Low Density Apolipoprotein II gene locus in transgenic mice

Nucleic Acids Res. 1993 Apr 11;21(7):1629-35. doi: 10.1093/nar/21.7.1629.

Abstract

We have examined the chicken Very Low Density Apolipoprotein II (apoVLDL II) gene locus in transgenic mice. A DNA fragment composed of the transcribed region, 16 kb of 5' flanking and 400 bp of 3' flanking sequences contained all the information sufficient for estrogen-inducible, liver-specific expression of the apoVLDL II gene. The far-upstream region contains a Negative Regulating Element coinciding with a DNaseI-hypersensitive site at -11 kb. In transgenic mice, the NRE at -11 kb is used for downregulating the expression to a lower maximum level. The NRE might be used for modulating apoVLDL II gene expression, and may be involved in the rapid shut-down of the expression after hormone removal.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apolipoproteins / genetics*
  • Base Sequence
  • Chickens
  • Deoxyribonuclease I
  • Estrogens / physiology
  • Gene Expression Regulation
  • Lipoproteins, VLDL / genetics*
  • Liver / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Transgenic
  • Molecular Sequence Data
  • Organ Specificity
  • Regulatory Sequences, Nucleic Acid

Substances

  • Apolipoproteins
  • Estrogens
  • Lipoproteins, VLDL
  • apolipoprotein VLDL II
  • Deoxyribonuclease I