Oligoclonal development of germinal centre cells as detected by anti-idiotype antibodies

Scand J Immunol. 1993 May;37(5):602-4. doi: 10.1111/j.1365-3083.1993.tb02578.x.

Abstract

Experimental models using immunization with known haptens have shown that germinal centres develop from only a limited number of precursor cells which expand in an oligoclonal fashion. Using a panel of antibodies raised against the idiotype of follicle centre cell lymphomas and known to be reactive only with a few normal B cells, we could demonstrate that individual clones can be identified within germinal centres of individual follicles of human lymph nodes and tonsils. Therefore also in humans in a non-experimental, natural situation, germinal centres expand in an oligoclonal fashion.

MeSH terms

  • Antibodies, Anti-Idiotypic*
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology
  • Child, Preschool
  • Humans
  • Immunoenzyme Techniques
  • Immunoglobulin Idiotypes / biosynthesis
  • Lymph Nodes / cytology
  • Lymph Nodes / immunology
  • Lymphocyte Activation / immunology
  • Lymphoid Tissue / cytology*
  • Lymphoid Tissue / immunology
  • Lymphoma, Follicular / immunology
  • Palatine Tonsil / cytology
  • Palatine Tonsil / immunology
  • Spleen / cytology
  • Spleen / immunology

Substances

  • Antibodies, Anti-Idiotypic
  • Immunoglobulin Idiotypes