Cloning and functional expression of a brain G-protein-coupled ATP receptor

FEBS Lett. 1993 Jun 14;324(2):219-25. doi: 10.1016/0014-5793(93)81397-i.

Abstract

A cDNA encoding a novel member of the G-protein-coupled receptor (GCR) superfamily, an ATP receptor, has been isolated from an embryonic chick whole brain cDNA library by hybridization screening. The encoded protein has a sequence of 362 amino acids (41 kDa) and shares no more than 27% amino acid identity with any known GCR. When expressed as a complementary RNA (cRNA) in Xenopus oocytes a slowly-developing inward current was observed in response to application of ATP. The pharmacology of this expressed protein defines it as a P2Y purinoceptor.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain Chemistry*
  • Chick Embryo
  • Cloning, Molecular
  • Electric Conductivity
  • GTP-Binding Proteins / metabolism*
  • Molecular Sequence Data
  • Oocytes
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Receptors, Purinergic / biosynthesis
  • Receptors, Purinergic / classification
  • Receptors, Purinergic / genetics*
  • Recombinant Proteins / biosynthesis
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription, Genetic
  • Xenopus

Substances

  • RNA, Messenger
  • Receptors, Purinergic
  • Recombinant Proteins
  • Adenosine Triphosphate
  • GTP-Binding Proteins