Abstract
PIP:
All subtypes of HIV-1 have been identified in Africa. The envelope glycoprotein of the HIV-1 is the most variable region of the virus, with the third variable region, including the V3 loop, a major target of vaccine research. This paper reports the identification of the HIV-1 subtypes present in 14 viral strains, isolated between 1984 and 1992 in South Africa. HIV-1 strains were isolated routinely at Tygerberg Hospital in the Western Cape region, with genomic DNA isolated from virus-infected cultures. After presenting the distance calculations and describing the tree constructions and bootstrap analysis, the authors emphasize the need for ongoing molecular epidemiological analysis of HIV-1 subtypes to track the current epidemic in South Africa. More rapid methods will facilitate subtyping to monitor the circulation and spread of HIV-1 subpopulations in the country.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Amino Acid Sequence
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Gene Products, env / genetics*
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HIV Envelope Protein gp120 / genetics
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HIV-1 / classification*
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HIV-1 / genetics
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HIV-1 / isolation & purification
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Humans
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Molecular Sequence Data
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Peptide Fragments / genetics
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Phylogeny
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Sequence Homology, Amino Acid
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South Africa
Substances
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Gene Products, env
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HIV Envelope Protein gp120
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HIV envelope protein gp120 (305-321)
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Peptide Fragments
Associated data
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GENBANK/L47608
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GENBANK/L48061
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GENBANK/L48062
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GENBANK/L48063
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GENBANK/L48064
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GENBANK/L48065
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GENBANK/L48066
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GENBANK/L48067
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GENBANK/L48068
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GENBANK/L48069
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GENBANK/L48070
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GENBANK/L48071
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GENBANK/L48072
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GENBANK/L48073
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GENBANK/U33769
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GENBANK/U33770
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GENBANK/U33771
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GENBANK/U33772
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GENBANK/U33773
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GENBANK/U33774
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GENBANK/U33775
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GENBANK/U33776
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GENBANK/U33777
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GENBANK/U33778
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GENBANK/U33779
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GENBANK/U33780
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GENBANK/U33781
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GENBANK/U33782