Abstract
We report on the purification of the full-length structural protein encoded by open reading frame 2 (ORF-2) of hepatitis E virus. The ORF-2 protein, expressed in Sf9 cells by using a recombinant baculovirus vector system, was successfully purified to homogeneity. Gel electrophoresis of the purified ORF-2 protein showed a single polypeptide of 75 kDa by Coomassie blue staining and by Western blot (immunoblot) analysis. We demonstrated that the partially purified ORF-2 protein could be used successfully in a sensitive and specific enzyme-linked immunosorbent assay for the detection of antibodies to hepatitis E virus.
Publication types
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Animals
-
Cell Line
-
Enzyme-Linked Immunosorbent Assay / methods*
-
Gene Expression
-
Genetic Vectors
-
Hepatitis Antibodies / blood
-
Hepatitis E / diagnosis
-
Hepatitis E / immunology
-
Hepatitis E virus / genetics*
-
Hepatitis E virus / immunology*
-
Humans
-
Immunoglobulin M / blood
-
Nucleopolyhedroviruses / genetics
-
Open Reading Frames
-
Protein Folding
-
Recombinant Proteins / genetics
-
Recombinant Proteins / immunology
-
Recombinant Proteins / isolation & purification
-
Spodoptera
-
Viral Structural Proteins / genetics*
-
Viral Structural Proteins / immunology*
-
Viral Structural Proteins / isolation & purification
Substances
-
Hepatitis Antibodies
-
Immunoglobulin M
-
Recombinant Proteins
-
Viral Structural Proteins