Direct database searching with MALDI-PSD spectra of peptides

P R Griffin; M J MacCoss; J K Eng; R A Blevins; J S Aaronson; J R Yates 3rd

doi:10.1002/rcm.1290091515

Direct database searching with MALDI-PSD spectra of peptides

Rapid Commun Mass Spectrom. 1995;9(15):1546-51. doi: 10.1002/rcm.1290091515.

Authors

P R Griffin¹, M J MacCoss, J K Eng, R A Blevins, J S Aaronson, J R Yates 3rd

Affiliation

¹ Department of Inflammation Research, Merck Research Laboratories, Rahway, NJ 07065-0900, USA.

PMID: 8652878
DOI: 10.1002/rcm.1290091515

Abstract

The analysis of matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) mass spectra of peptides by using the cross-correlation method for database searching is illustrated. MALDI-PSD mass spectra are shown to contain sufficient fragmentation information to uniquely identify the correct amino acid sequence from large protein databases (approximately 160,000 entries). A search employing the MALDI-PSD mass spectrum of a phosphorylated peptide that correctly identifies the amino acid sequence and the site of phosphorylation is also illustrated.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Databases, Factual*
Gas Chromatography-Mass Spectrometry / methods*
Humans
Molecular Sequence Data
Peptides / analysis*
Phosphopeptides / analysis

Substances

Peptides
Phosphopeptides