Abstract
The polymorphic Bcl I site in the human ucp gene associated to percentage fat gain over time in the Québec Family Study cohort (Oppert et al. Int J Obesity 1994; 18: 526-531) has been positioned to the 5'-flanking region. This polymorphism results from a unique A/G mutation. Oligonucleotides used to amplify the polymorphic region, and allowing future studies of any cohort of patients, are described.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Carrier Proteins / genetics*
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DNA / chemistry*
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Deoxyribonucleases, Type II Site-Specific / metabolism*
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Humans
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Ion Channels
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Membrane Proteins / genetics*
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Mitochondrial Proteins
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Molecular Sequence Data
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Obesity / genetics
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Point Mutation*
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Polymorphism, Restriction Fragment Length*
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Restriction Mapping
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Uncoupling Protein 1
Substances
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Carrier Proteins
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Ion Channels
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Membrane Proteins
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Mitochondrial Proteins
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Uncoupling Protein 1
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DNA
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endodeoxyribonuclease BclI
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Deoxyribonucleases, Type II Site-Specific