Abstract
The major mRNP protein of rabbit reticulocytes, p50, belonging to the family of Y-box transcription factors has been expressed in E. coli. The isolation procedure of the recombinant protein has been described. The recombinant protein is similar to the protein isolated from rabbit mRNPs in SDS-PAGE mobility and interaction with specific antibodies. Similar to the natural protein, the recombinant protein forms homooligomeric complexes with a molecular mass of about 800 kDa, binds to the alpha-globin RNA and double-stranded DNA containing the Y-box. Both proteins can be phosphorylated in vitro.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Base Sequence
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Biopolymers
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Chromatography, Gel
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Chromatography, Ion Exchange
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Cloning, Molecular
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / genetics
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Molecular Sequence Data
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Nucleic Acids / metabolism
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Oligodeoxyribonucleotides
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Phosphorylation
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Protein Binding
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Rabbits
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Ribonucleoproteins / genetics*
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Ribonucleoproteins / isolation & purification
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Ribonucleoproteins / metabolism
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Transcription Factors / genetics*
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Transcription Factors / isolation & purification
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Transcription Factors / metabolism
Substances
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Biopolymers
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Nucleic Acids
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Oligodeoxyribonucleotides
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Recombinant Proteins
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Ribonucleoproteins
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Transcription Factors