Culture is one of the important methods for detecting Helicobacter pylori(Hp). Culture method is generally lower sensitive in comparison with other Hp detection methods. However, culture is necessary for determination of MIC and analysis of bacteriological characteristics. Several culture media are now commercially available for Hp. In this study, we compared commercial media with our original medium. Original medium for Hp culture was most sensitive and showed no false-positive results compared with other commercial media.