Purification of overproduced Escherichia coli RNA polymerase sigma factors by solubilizing inclusion bodies and refolding from Sarkosyl

Methods Enzymol. 1996:273:145-9. doi: 10.1016/s0076-6879(96)73014-8.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / isolation & purification*
  • Bacteriolysis / drug effects
  • Chromatography, High Pressure Liquid
  • DNA-Directed RNA Polymerases / chemistry*
  • Detergents / pharmacology
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Inclusion Bodies / chemistry
  • Protein Folding
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / isolation & purification*
  • Sarcosine / analogs & derivatives
  • Sarcosine / pharmacology
  • Sigma Factor / biosynthesis
  • Sigma Factor / chemistry
  • Sigma Factor / genetics
  • Sigma Factor / isolation & purification*
  • Solubility

Substances

  • Bacterial Proteins
  • Detergents
  • Recombinant Fusion Proteins
  • Sigma Factor
  • sarkosyl
  • DNA-Directed RNA Polymerases
  • Sarcosine