Abstract
Pulmonary cryptococcosis was diagnosed by nested PCR. Extraction of DNA was performed by mechanical destruction of the capsules of Cryptococcus neoformans by the glass bead technique. Nested PCR was positive for 4 of 5 culture-positive specimens but negative for 1 culture-positive specimen, 10 culture-negative specimens, and 1 specimen with undetermined culture results.
MeSH terms
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Base Sequence
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Cryptococcosis / diagnosis*
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Cryptococcosis / microbiology*
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Cryptococcus neoformans / genetics*
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Cryptococcus neoformans / isolation & purification*
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DNA Primers / genetics
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DNA, Fungal / genetics
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DNA, Fungal / isolation & purification
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Diagnostic Errors
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Evaluation Studies as Topic
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Genes, Fungal*
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Humans
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Lung Diseases, Fungal / diagnosis*
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Lung Diseases, Fungal / microbiology*
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Mycology / methods
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Polymerase Chain Reaction / methods