Identification of novel exon-deleted progesterone receptor variant mRNAs in human breast tissue

Biochem Biophys Res Commun. 1996 Nov 1;228(1):63-8. doi: 10.1006/bbrc.1996.1616.

Abstract

Using an approach based on the co-amplification of wild-type and exon deleted progesterone receptor (PR) variant cDNAs, we identified exon-deleted PR variant mRNAs in both normal and neoplastic human breast tissues. Several naturally occurring variants, whose sequences revealed precise whole exon deletions, may encode putative PR-like proteins which lack some functional domains of the wild-type PR molecule. We suggest that these PR variant proteins could have a pathophysiological role in progestin action, as suggested for estrogen receptor variant proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Breast / chemistry*
  • Breast Neoplasms / chemistry*
  • Breast Neoplasms / genetics
  • Carcinoma, Ductal, Breast / chemistry
  • Carcinoma, Ductal, Breast / genetics
  • Exons*
  • Female
  • Genetic Variation
  • Humans
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics*
  • Receptors, Estrogen / analysis
  • Receptors, Progesterone / genetics*
  • Sequence Deletion*
  • Tumor Cells, Cultured

Substances

  • RNA, Messenger
  • Receptors, Estrogen
  • Receptors, Progesterone