Data from nearly 2500 British Caucasians, profiled using an STR quadruplex, have been analysed. The data came from several laboratories and represent samples from different geographical distributions. Analysis of the combined files shows that previous reports of failed independence tests were the results of sampling effects. A further convincing proof is given of the robustness of the statistical methods used to estimate evidential value in casework. Comparisons between different samples show that regional effects between Scotland and the South of England have no importance from the forensic viewpoint.