Molecular cloning of two new HLA-C alleles: Cw*1801 and Cw*0706

Tissue Antigens. 1996 Dec;48(6):698-702. doi: 10.1111/j.1399-0039.1996.tb02694.x.

Abstract

Nucleotide sequence analysis of the HLA-C alleles of the GB92 cell line, heterozygous for B*8101 and B*4407, revealed the existence of two new allelic variants: Cw*1801 and Cw*0706. The former allele, initially detected as a PCR-SSP variant, displays a hybrid aspect, sharing sequence motifs with Cw*07 at exons 1 and 2, and with Cw*04 at distal exons. In serological assays, Cw*1801 is only recognized by some cross-reactive sera. Cw*0706 shows a primary structure closely related to previously known Cw7 alleles, but carries new sequence motifs at its 3'-end. Preliminary data indicate that Cw*1801 is associated to B*8101 and that Cw*0706, B*4407 could account for a part of the Cw7, B44 haplotypes observed in African populations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA
  • HLA-B Antigens / genetics
  • HLA-C Antigens / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Transcription, Genetic

Substances

  • HLA-B Antigens
  • HLA-C Antigens
  • HLA-C*70 antigen
  • DNA

Associated data

  • GENBANK/X96582
  • GENBANK/X97321