Expression, purification, and characterization of vaccinia virus-encoded RNA and poly(A) polymerases

Methods Enzymol. 1996:275:208-27. doi: 10.1016/s0076-6879(96)75014-0.

Authors

P Gershon¹, B Moss

Affiliation

¹ Department of Biochemistry and Biophysics, Institute of Biosciences and Technology, Texas A&M University, College Station 77843, USA.

PMID: 9026640
DOI: 10.1016/s0076-6879(96)75014-0

No abstract available

MeSH terms

Chromatography, DEAE-Cellulose / methods
Chromatography, Gel / methods
Chromatography, Ion Exchange / methods
DNA-Directed RNA Polymerases / chemistry
DNA-Directed RNA Polymerases / isolation & purification*
DNA-Directed RNA Polymerases / metabolism*
HeLa Cells
Humans
Indicators and Reagents
Kinetics
Macromolecular Substances
Polynucleotide Adenylyltransferase / chemistry
Polynucleotide Adenylyltransferase / isolation & purification*
Polynucleotide Adenylyltransferase / metabolism*
RNA, Messenger / metabolism
RNA, Viral / metabolism
Recombinant Proteins / chemistry
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
Transcription, Genetic
Vaccinia virus / enzymology*
Virion / enzymology

Substances

Indicators and Reagents
Macromolecular Substances
RNA, Messenger
RNA, Viral
Recombinant Proteins
Polynucleotide Adenylyltransferase
DNA-Directed RNA Polymerases