Methanoplanus petrolearius sp. nov., a novel methanogenic bacterium from an oil-producing well

FEMS Microbiol Lett. 1997 Feb 1;147(1):51-6. doi: 10.1111/j.1574-6968.1997.tb10219.x.

Abstract

A disc-shaped methanogenic bacterium designated strain SEBR 4847T (T = type strain) was isolated from a sample collected from an African offshore oil field. Strain SEBR 4847T was non-motile, had a G + C content of 50 mol% and produced methane from H2 + CO2, formate, and CO2 + propanol. Strain SEBR 4847T grew optimally at 37 degrees C; no growth was observed at 25 degrees C or 45 degrees C. It grew in the presence of up to 50 g/l NaCl; 10-30 g/l was required for optimal growth. The optimum pH for growth was 7.0. Doubling time was about 10 h under optimal conditions. Based on 16S rRNA sequence analysis, the isolate was identified as a new species of the genus Methanoplanus and designated Methanoplanus petrolearius sp. nov. The type strain is SEBR 4847T (= OCM 486).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques
  • Classification
  • Culture Media
  • Cytosine
  • DNA, Bacterial / analysis
  • Euryarchaeota / classification*
  • Euryarchaeota / cytology
  • Euryarchaeota / isolation & purification*
  • Guanine
  • Guinea
  • Molecular Sequence Data
  • Oils
  • RNA, Bacterial / analysis
  • RNA, Ribosomal, 16S / analysis
  • Sequence Analysis, RNA
  • Soil Microbiology*

Substances

  • Culture Media
  • DNA, Bacterial
  • Oils
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Guanine
  • Cytosine

Associated data

  • GENBANK/U76631