Abstract
Bacterial vaccine, as generated by heat-inactivated Clostridium butyricum cells, displayed antitumor activity against sarcoma 180 in DDY mice and antimetastatic activity against B16-F10 melanoma in BDF1 mice. According to our results, the vaccine has no direct growth inhibitory effect toward the tumor cell lines tested in this study. The vaccine increased gamma-interferon production, elicited delayed type hypersensitivity reaction, and enhanced IgM antibody formation and mitogenicity. The phagocytic activity of macrophage and killing activity of NK cells from mice were enhanced in a dose-dependent manner by stimulating with the heat-inactivated vaccine. Among those responses in the mice treated with CB, elevated NK cell activity may play a prominent role in manifesting antitumor activity in the B16-F10 metastasis experiment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / pharmacology
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Adjuvants, Immunologic / therapeutic use*
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Animals
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Bacterial Vaccines / pharmacology
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Bacterial Vaccines / therapeutic use*
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Clostridium / immunology*
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Cytotoxicity, Immunologic / drug effects
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Immunity, Cellular / drug effects
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Immunoglobulin M / biosynthesis
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Interferon Inducers / pharmacology
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Interferon Inducers / therapeutic use*
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Interferon-gamma / biosynthesis
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Killer Cells, Natural / drug effects
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Lung Neoplasms / prevention & control
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Lung Neoplasms / secondary
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Lymphocyte Activation / drug effects
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Macrophage Activation / drug effects
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Male
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Melanoma, Experimental / drug therapy
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Melanoma, Experimental / pathology
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Melanoma, Experimental / prevention & control
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Melanoma, Experimental / secondary
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Mice
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Neoplasm Transplantation
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Phagocytosis / drug effects
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Sarcoma 180 / therapy*
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Specific Pathogen-Free Organisms
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Tumor Cells, Cultured
Substances
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Adjuvants, Immunologic
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Bacterial Vaccines
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Immunoglobulin M
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Interferon Inducers
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Interferon-gamma