False-positive results of genetic testing in cystic fibrosis

J Pediatr. 1997 Apr;130(4):658-60. doi: 10.1016/s0022-3476(97)70255-5.

Abstract

We describe a patient in whom newborn immunoreactive trypsin screening and mutation analysis suggested a diagnosis of cystic fibrosis; however, the clinical course and sweat test results were not consistent with the diagnosis. Direct sequencing of the patient's genomic DNA showed compound heterozygosity for delta F508 and F508C, a polymorphism not associated with clinical disease.

Publication types

  • Case Reports

MeSH terms

  • Cystic Fibrosis / diagnosis*
  • Cystic Fibrosis Transmembrane Conductance Regulator / genetics
  • DNA Mutational Analysis
  • False Positive Reactions
  • Genetic Techniques
  • Humans
  • Infant, Newborn
  • Male
  • Point Mutation
  • Polymerase Chain Reaction
  • Trypsin / analysis

Substances

  • CFTR protein, human
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Trypsin