Effects of estrogen on skeletal myoblast growth

Biochem Biophys Res Commun. 1997 Mar 17;232(2):373-8. doi: 10.1006/bbrc.1997.6223.

Abstract

To determine the role of estrogen in skeletal muscle growth, we investigated estrogen receptor-mediated effects on proliferation in skeletal myoblasts. In L6, C2C12 and Sol8 myoblasts estrogen receptor was demonstrated by immunoblotting, immunofluorescence microscopy and transfection studies. Estrone induced a significant increase in myoblast growth whereas 17 beta-estradiol had no effect. Furthermore in L6-cells estrone (c-fos: 3.9-fold, egr-1: 4.6-fold) induced immediate-early gene induction significantly stronger than 17 beta-estradiol (c-fos: 1.7-fold, egr-1: 2.3-fold; p < 0.05). Skeletal myoblasts express functional estrogen receptors. Estrogens differ in the activation of skeletal myoblast growth and immediate-early gene induction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Division / drug effects
  • Cell Line
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics
  • Early Growth Response Protein 1
  • Estrogens / metabolism
  • Estrogens / pharmacology*
  • Estrogens / physiology
  • Gene Expression Regulation / drug effects
  • Immediate-Early Proteins*
  • Mice
  • Muscle Development*
  • Muscle, Skeletal / cytology
  • Muscle, Skeletal / drug effects*
  • Muscle, Skeletal / growth & development*
  • Rats
  • Receptors, Estrogen / metabolism
  • Receptors, Estrogen / physiology
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Transcriptional Activation

Substances

  • DNA-Binding Proteins
  • Early Growth Response Protein 1
  • Egr1 protein, mouse
  • Egr1 protein, rat
  • Estrogens
  • Immediate-Early Proteins
  • Receptors, Estrogen
  • Transcription Factors