K(ATP) channels contribute to the cardioprotection of preconditioning independent of anaesthetics in rabbit hearts

J Mol Cell Cardiol. 1997 Apr;29(4):1267-76. doi: 10.1006/jmcc.1996.0364.

Abstract

The contribution of ATP sensitive potassium (K(ATP)) channels to the infarct-size limiting effect of preconditioning is considered to be anaesthetic-dependent in the rabbit heart. It has previously been reported that ischaemic preconditioning prevents ischaemia-induced reductions in activities of sarcolemmal adenylate cyclase (AC) and Na+, K(+)-ATPase. Anaesthetic dependency of the role of K(ATP) channels in the preservation of these enzyme activities, induced by ischaemic preconditioning, as well as that induced by activation of A1-adenosine receptors, was examined in rabbits anaesthetized with either pentobarbital or ketamine-xylazine and subjected to 20 min of regional ischaemia. Adenylate cyclase and Na+, K(+)-ATPase activities were lower in the ischaemic than in the non-ischaemic region of the hearts in control rabbits, but not in animals subjected to ischaemic preconditioning, or those pretreated with the A1-adenosine receptor agonist R(-)-N6-(2-phenylisopropyl) adenosine. The protective effects of both ischaemic preconditioning and A1-adenosine receptor activation were prevented by 6 mg/kg, but not 3 mg/kg, of the K(ATP) channel blocker, glibenclamide, in rabbits anaesthetized with pentobarbital, while these effects were prevented by 3 mg/kg of the blocker in rabbits anaesthetized with ketamine-xylazine. Moreover, K(ATP) channel opener, cromakalim, prevented the ischaemia-induced decreases in enzymatic activities in rabbits subjected to either type of anaesthesia. Thus, although the antagonistic effect of glibenclamide is blunted under pentobarbital, compared to ketamine-xylazine anaesthesia, K(ATP) channels contribute to preservative actions independent of the type of anaesthesia in the rabbit heart.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Adenylyl Cyclases / drug effects
  • Adenylyl Cyclases / metabolism
  • Anesthetics / pharmacology*
  • Animals
  • Cardiotonic Agents / pharmacology
  • Female
  • Hemodynamics
  • Ischemic Preconditioning, Myocardial*
  • Isoproterenol / pharmacology
  • Ketamine / pharmacology
  • Myocardial Ischemia / metabolism
  • Myocardium / metabolism*
  • Pentobarbital / pharmacology
  • Potassium Channels / metabolism*
  • Rabbits
  • Receptors, Purinergic P1 / metabolism
  • Sarcolemma / metabolism*
  • Sodium-Potassium-Exchanging ATPase / metabolism
  • Xylazine / pharmacology

Substances

  • Anesthetics
  • Cardiotonic Agents
  • Potassium Channels
  • Receptors, Purinergic P1
  • Xylazine
  • Ketamine
  • Adenosine Triphosphate
  • Adenylyl Cyclases
  • Sodium-Potassium-Exchanging ATPase
  • Pentobarbital
  • Isoproterenol