Image analysis of nuclear/cytoplasmic ratio in cervical smears to discriminate three grades of cervical intraepithelial neoplasia

Acta Cytol. 1997 May-Jun;41(3):744-8. doi: 10.1159/000332697.

Abstract

Objective: To suggest which system of cytologic classification (Bethesda System or World Health Organization [WHO]) of cervical lesions is more adequate for the Public Health Service of São Paulo State, Brazil, based on morphometric study with the Jandel Videoanalysis (JAVA) System.

Study design: The study comprised groups of typical smears: cervical intraepithelial neoplasia (CIN 1) separated into two subgroups: cytopathic effects of human papillomavirus associated or not with dyskaryosis, CIN 2 and CIN 3. The JAVA system of image analysis was used to determine the nuclear/cytoplasmic (N/C) ratio in abnormal cells from each group.

Results: Significant differences were detected between the three grades of CIN and between the two subgroups of CIN 1.

Conclusion: Although image analysis is not applicable to large-scale population screening of cervical smears, its use in the present study favored the three-grade cytologic classification (WHO). In addition, the three-grade classification offers the clinician more options for treatment. Considering the clinical-laboratory characteristics of our public health service, the three-grade classification is more adequate.

MeSH terms

  • Cell Nucleus / pathology
  • Cytoplasm / pathology
  • Female
  • Humans
  • Image Processing, Computer-Assisted
  • Papillomaviridae
  • Papillomavirus Infections / pathology
  • Tumor Virus Infections / pathology
  • Uterine Cervical Dysplasia / classification*
  • Uterine Cervical Dysplasia / diagnosis*
  • Uterine Cervical Neoplasms / classification*
  • Uterine Cervical Neoplasms / diagnosis*