Treatment with recombinant human erythropoietin (rHuEpo) in a patient with paroxysmal nocturnal haemoglobinuria: evaluation of membrane proteins CD55 and CD59 with cytofluorometric assay

Br J Haematol. 1997 Jun;97(3):586-8. doi: 10.1046/j.1365-2141.1997.912908.x.

Abstract

We describe a 28-year-old man with paroxysmal nocturnal haemoglobinuria (PNH) and a high transfusion requirement. Prior to and during therapy with recombinant human erythropoietin (rHuEpo), we evaluated the levels of 'decay-accelerating-factor', CD55, and 'membrane-inhibitor-of-reactive-lysis', CD59, as markers of the disease, whilst CD58, a marker present on leucocytes, was utilized to monitor normal haemopoietic activity. The patient became transfusion independent 1 month after beginning rHuEpo and remains well. The analysis of CD55, CD59 and CD58 suggests that the efficacy of rHuEpo was due to a selective rHuEpo action on normal erythroid clones.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • CD55 Antigens / metabolism*
  • CD58 Antigens / metabolism
  • CD59 Antigens / metabolism*
  • Erythrocytes / metabolism
  • Erythropoietin / therapeutic use*
  • Flow Cytometry
  • Hemoglobinuria, Paroxysmal / therapy*
  • Humans
  • Male

Substances

  • CD55 Antigens
  • CD58 Antigens
  • CD59 Antigens
  • Erythropoietin