Transformation-related expression of a low-molecular-mass tropomyosin isoform TM5/TM30nm in transformed rat fibroblastic cell lines

K Miyado; M Sato; S Taniguchi

doi:10.1007/BF01438309

Transformation-related expression of a low-molecular-mass tropomyosin isoform TM5/TM30nm in transformed rat fibroblastic cell lines

J Cancer Res Clin Oncol. 1997;123(6):331-6. doi: 10.1007/BF01438309.

Authors

K Miyado¹, M Sato, S Taniguchi

Affiliation

¹ Department of Molecular and Developmental Science, Tokai University, Ischara, Japan.

PMID: 9222299
DOI: 10.1007/BF01438309

Abstract

We cloned a full-length rat TM5/TM30nm cDNA. Using this cDNA as a probe, we demonstrated that expression of TM5/TM30nm mRNA was higher in the tumorigenic rat fibroblastic cell lines SR-3Y1-2 and fos-SR-3Y1-202 than in the normal cell line 3Y1. High expression of TM5/TM30nm protein in SR-3Y1-2 and fos-SR-3Y1-202 cells was also detected by Western blot analysis using anti-TM5/TM30nm antiserum. In addition, the cellular localization of this protein differed between 3Y1 cells and tumorigenic ones. These findings suggest that TM5/TM30nm is involved in malignant transformation of rat fibroblastic cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cell Line
Cell Transformation, Neoplastic*
Fibroblasts
Molecular Sequence Data
Molecular Weight
RNA, Messenger / analysis
Rats
Tropomyosin / analysis*
Tropomyosin / genetics

Substances

RNA, Messenger
Tropomyosin