[Radioimmunologic methods for determining cortisol binding globulin-CBG in human serum]

Ginekol Pol. 1996 Nov;67(11):545-7.
[Article in Polish]

Abstract

The authors described double antibodies radioimmunoassay of human cortisol binding globulin serum level in support of previous isolated and purified trancortin and anti-transcortin antibodies produced by rabbit immunization. Antigen was iodinated by chloraminet method and purification on Ultragel AcA 54 column. Standard curve had specific Bx/Box100%. Using described method investigation of transcortin globulin serum level is precise and repetitive.

Publication types

  • Clinical Trial
  • Controlled Clinical Trial
  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / blood*
  • Carrier Proteins / isolation & purification
  • Female
  • Humans
  • Male
  • Pregnancy
  • Pregnancy Trimester, Second / blood*
  • Pregnancy Trimester, Third / blood*
  • Radioimmunoassay / methods*
  • Radioimmunoassay / standards
  • Reference Values
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Carrier Proteins
  • cortisol binding globulin