A CXCR4/CD4 pseudotype rhabdovirus that selectively infects HIV-1 envelope protein-expressing cells

Cell. 1997 Sep 5;90(5):841-7. doi: 10.1016/s0092-8674(00)80349-9.

Abstract

We show that a cellular virus receptor functions in the envelope of a virus, allowing selective infection of cells displaying the receptor ligand. A G-deficient rabies virus (RV) pseudotyped with CD4- and CXCR4-derived proteins selectively infected cells expressing HIV-1 envelope protein. Envelope protein or CD4 antibodies blocked virus entry. Pseudotype virus formation was most efficient with chimeric receptor proteins possessing the cytoplasmic tail of the RV G spike protein (CXCR4-RV and CD4-RV). While CXCR4-RV was incorporated when expressed alone, CD4-RV incorporation required CXCR4-RV as a carrier protein, indicating a mechanism by which oligomeric surface proteins are sorted into the RV envelope. Viral vectors bearing virus receptors in their envelope may be useful reagents for targeting virus-infected cells in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CD4 Antigens / metabolism*
  • Carrier Proteins / metabolism
  • Cricetinae
  • GTP-Binding Proteins / metabolism
  • HIV Infections / therapy
  • HIV Infections / virology*
  • HIV-1 / growth & development
  • HIV-1 / physiology*
  • HeLa Cells
  • Humans
  • Kidney / cytology
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Receptors, CXCR4
  • Receptors, HIV / metabolism*
  • Recombinant Fusion Proteins / physiology
  • Rhabdoviridae / chemistry
  • Rhabdoviridae / growth & development
  • Rhabdoviridae / physiology*
  • Viral Envelope Proteins / metabolism*
  • Virus Latency / physiology
  • Virus Replication / physiology

Substances

  • CD4 Antigens
  • Carrier Proteins
  • Membrane Proteins
  • Receptors, CXCR4
  • Receptors, HIV
  • Recombinant Fusion Proteins
  • Viral Envelope Proteins
  • GTP-Binding Proteins