Abstract
A bacterioferritin (BFR) deletion mutant of Brucella melitensis 16M was generated by gene replacement. The deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pBBR-bfr. The survival and growth of the mutant, B. melitensis PAD 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (MDM). These results suggest that BFR is not essential for the intracellular survival of B. melitensis in human MDM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins*
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Brucella melitensis / genetics
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Brucella melitensis / growth & development
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Brucella melitensis / pathogenicity*
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Cytochrome b Group / genetics*
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Ferritins / genetics*
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Gene Deletion
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Genetic Complementation Test
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Humans
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Macrophages / immunology*
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Macrophages / microbiology
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Monocytes / immunology*
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Monocytes / microbiology
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Mutation
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Oxidative Stress
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Species Specificity
Substances
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Bacterial Proteins
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Cytochrome b Group
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Ferritins
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bacterioferritin