Improved translational efficiency of subtilisin YaB gene with different initiation codons in Bacillus subtilis and alkalophilic Bacillus YaB

C M Yeh; H K Chang; H M Hsieh; K Yoda; M Yamasaki; Y C Tsai

doi:10.1046/j.1365-2672.1997.00306.x

Improved translational efficiency of subtilisin YaB gene with different initiation codons in Bacillus subtilis and alkalophilic Bacillus YaB

J Appl Microbiol. 1997 Dec;83(6):758-63. doi: 10.1046/j.1365-2672.1997.00306.x.

Authors

C M Yeh¹, H K Chang, H M Hsieh, K Yoda, M Yamasaki, Y C Tsai

Affiliation

¹ Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan, Republic of China.

PMID: 9449813
DOI: 10.1046/j.1365-2672.1997.00306.x

Abstract

The ale gene specifying the subtilisin YaB produced by alkalophilic Bacillus YaB, has an unusual start codon UUG. Changing this codon to AUG and GUG increased expression of the ale gene in B. subtilis DB104 and in an ale deficient mutant strain YaB-DEC4. The relative translational efficiency order of the three initiation codons is AUG > GUG > UUG in B. subtilis DB104 and in YaB-DEC4. These data suggest that the preferred initiation codon is AUG for ale gene expression in Bacillus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / genetics*
Codon*
Pancreatic Elastase / genetics*
Protein Biosynthesis*
Subtilisins / genetics*

Substances

Codon
Subtilisins
alkaline elastase
Pancreatic Elastase