Abstract
The subcellular localization of the mGlu4a metabotropic glutamate receptor was investigated in rat cerebellum. At the light microscopical level, strong mGlu4a immunoreactivity was found in the molecular layer. A post-embedding immunogold method for electron microscopy revealed gold particles at the presynaptic sites of synapses made by parallel fiber terminals with dendritic spines of Purkinje cells. These observations support electrophysiological evidence indicating an autoreceptor function of mGlu4 receptors at these synapses.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibody Specificity
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Cerebellar Cortex / metabolism*
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Cerebellar Cortex / ultrastructure*
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Dendrites / metabolism
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Dendrites / ultrastructure
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Male
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Microscopy, Immunoelectron
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Molecular Sequence Data
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Presynaptic Terminals / metabolism
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Presynaptic Terminals / ultrastructure
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Purkinje Cells / metabolism
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Purkinje Cells / ultrastructure
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Rats
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Rats, Sprague-Dawley
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Receptors, Metabotropic Glutamate / genetics
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Receptors, Metabotropic Glutamate / immunology
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Receptors, Metabotropic Glutamate / metabolism*
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Synapses / metabolism
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Synapses / ultrastructure
Substances
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Peptide Fragments
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Receptors, Metabotropic Glutamate
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metabotropic glutamate receptor 4