PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum beta-lactamase and to identify different SHV genes within the same strain

F H M'Zali; J Heritage; D M Gascoyne-Binzi; A M Snelling; P M Hawkey

doi:10.1093/jac/41.1.123

PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum beta-lactamase and to identify different SHV genes within the same strain

J Antimicrob Chemother. 1998 Jan;41(1):123-5. doi: 10.1093/jac/41.1.123.

Authors

F H M'Zali¹, J Heritage, D M Gascoyne-Binzi, A M Snelling, P M Hawkey

Affiliation

¹ Department of Microbiology, University of Leeds, UK.

PMID: 9511048
DOI: 10.1093/jac/41.1.123

Abstract

The PCR single strand conformational polymorphism (PCR-SSCP) technique described to identify mutants of the SHV beta-lactamases was extended to identify an SHV-7 type beta-lactamase. This was found in a strain of Klebsiella pneumoniae, the first recorded isolate in the UK to produce this type of enzyme. We also demonstrate that PCR-SSCP can be used to identify more than one SHV beta-lactamase gene in a single strain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
DNA, Bacterial / chemistry
Genes, Bacterial
Klebsiella pneumoniae / drug effects*
Klebsiella pneumoniae / enzymology
Microbial Sensitivity Tests
Nucleic Acid Conformation
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
DNA, Bacterial
beta-Lactamases