Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA

Appl Environ Microbiol. 1998 Apr;64(4):1550-4. doi: 10.1128/AEM.64.4.1550-1554.1998.

Abstract

The ability of Acinetobacter sp. strain BD413(pFG4 delta nptII) to take up and integrate transgenic plant DNA based on homologous recombination was studied under optimized laboratory conditions. Restoration of nptII, resulting in kanamycin-resistant transformants, was observed with plasmid DNA, plant DNA, and homogenates carrying the gene nptII. Molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter / genetics*
  • Base Sequence
  • Chenopodiaceae / genetics*
  • Chenopodiaceae / microbiology*
  • Chromosome Mapping
  • DNA Primers / genetics
  • DNA, Plant / genetics*
  • DNA, Recombinant / genetics
  • Gene Transfer Techniques
  • Kanamycin Resistance / genetics
  • Plants, Genetically Modified
  • Plasmids / genetics
  • Polymerase Chain Reaction
  • Transformation, Genetic*

Substances

  • DNA Primers
  • DNA, Plant
  • DNA, Recombinant