Caspase 7-induced cleavage of kinectin in apoptotic cells

FEBS Lett. 1998 Sep 25;436(1):51-4. doi: 10.1016/s0014-5793(98)01095-3.

Abstract

Kinectin has been characterized as the first known receptor for the molecular motor kinesin, which is critically involved in microtubule-based vesicle transport and membrane trafficking. Here we identify kinectin as a target for caspase-mediated proteolysis during apoptosis. Treatment of cells with diverse apoptotic stimuli including TNF, anti-Fas, anticancer drugs, gamma-radiation or ceramide leads to rapid proteolytic cleavage of the 160-kDa form of kinectin to a 120-kDa fragment. Evidence is provided that kinectin cleavage is mediated by caspase 7.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Chloromethyl Ketones / pharmacology
  • Antibodies / pharmacology
  • Apoptosis / drug effects
  • Apoptosis / radiation effects
  • Caspase 7
  • Caspases / drug effects
  • Caspases / genetics
  • Caspases / metabolism*
  • Cell-Free System
  • Ceramides / pharmacology
  • Cisplatin / pharmacology
  • Cysteine Proteinase Inhibitors / pharmacology
  • Daunorubicin / pharmacology
  • Etoposide / pharmacology
  • HeLa Cells / drug effects
  • HeLa Cells / metabolism
  • HeLa Cells / radiation effects
  • Humans
  • Jurkat Cells / drug effects
  • Jurkat Cells / metabolism
  • Jurkat Cells / radiation effects
  • Membrane Proteins*
  • Receptors, Cell Surface / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • fas Receptor / immunology
  • fas Receptor / metabolism

Substances

  • Amino Acid Chloromethyl Ketones
  • Antibodies
  • Ceramides
  • Cysteine Proteinase Inhibitors
  • KTN1 protein, human
  • Membrane Proteins
  • N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone
  • Receptors, Cell Surface
  • Recombinant Proteins
  • fas Receptor
  • Etoposide
  • CASP7 protein, human
  • Caspase 7
  • Caspases
  • Cisplatin
  • Daunorubicin