RNA polymerase II elongation complexes containing the Cockayne syndrome group B protein interact with a molecular complex containing the transcription factor IIH components xeroderma pigmentosum B and p62

J Biol Chem. 1998 Oct 23;273(43):27794-9. doi: 10.1074/jbc.273.43.27794.

Abstract

Transcription factor IIH (TFIIH) is involved both in transcription initiation by RNA polymerase II and in nucleotide excision-repair. Nucleotide excision-repair occurs at higher rates in transcriptionally active regions of the genome. Genetic studies indicate that this transcription-coupled repair is dependent on the Cockayne syndrome group A and B proteins, as well as TFIIH subunits. Previous work indicated that Cockayne syndrome group B interacts with RNA polymerase II molecules engaged in ternary complexes containing DNA and RNA. Evidence presented here indicates that this complex can interact with a factor containing the TFIIH core subunits p62 and xeroderma pigmentosum subunit B/excision repair cross-complementing 3. The targeting of TFIIH or a TFIIH-like repair factor to transcriptionally active DNA indicates a potential mechanism for transcription-coupled repair in human cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cockayne Syndrome
  • DNA Helicases / metabolism*
  • DNA Repair Enzymes
  • DNA Repair*
  • DNA-Binding Proteins / metabolism*
  • Humans
  • Models, Genetic
  • Poly-ADP-Ribose Binding Proteins
  • Protein Binding
  • RNA Polymerase II / metabolism*
  • Transcription Factor TFIIH
  • Transcription Factors / metabolism*
  • Transcription Factors, TFII*
  • Transcription, Genetic*
  • Xeroderma Pigmentosum

Substances

  • DNA-Binding Proteins
  • Poly-ADP-Ribose Binding Proteins
  • Transcription Factors
  • Transcription Factors, TFII
  • XPBC-ERCC-3 protein
  • Transcription Factor TFIIH
  • RNA Polymerase II
  • DNA Helicases
  • ERCC6 protein, human
  • DNA Repair Enzymes