Localization of HuC (ELAVL3) to chromosome 19p13.2 by fluorescence in situ hybridization utilizing a novel tyramide labeling technique

Genomics. 1998 Nov 1;53(3):296-9. doi: 10.1006/geno.1998.5468.

Abstract

HuC is a neural-specific member of the Elav family of RNA-binding proteins. This highly conserved gene family plays a crucial role in neurogenesis, and HuC (HGMW-approved symbol ELAVL3) is expressed at an early stage of neural development. Using a novel tyramide fluorescence in situ hybridization (T-FISH) technique, we localized HuC to chromosome 19p13.2. This localization was confirmed by radiation hybrid mapping and coincides with that of HuR (HGMW-approved symbol ELAVL1), another elav family member. Dual T-FISH analysis with HuC and HuR probes, however, indicated distinct loci, with HuC being centromeric to HuR. This study demonstrates the utility of T-FISH in colocalizing two genes on the same chromosomal preparation using only biotinylated probes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Surface / genetics*
  • Base Sequence
  • Chromosome Mapping / methods*
  • Chromosomes, Human, Pair 19 / genetics*
  • DNA Primers / genetics
  • ELAV Proteins
  • ELAV-Like Protein 1
  • ELAV-Like Protein 3
  • Fluorescent Dyes
  • Humans
  • Hybrid Cells
  • In Situ Hybridization, Fluorescence / methods*
  • Nerve Tissue Proteins / genetics*
  • RNA-Binding Proteins / genetics

Substances

  • Antigens, Surface
  • DNA Primers
  • ELAV Proteins
  • ELAV-Like Protein 1
  • ELAV-Like Protein 3
  • ELAVL1 protein, human
  • ELAVL3 protein, human
  • Fluorescent Dyes
  • Nerve Tissue Proteins
  • RNA-Binding Proteins