Rapid PCR detection of Helicobacter pylori-associated virulence and resistance genes directly from gastric biopsy material

J Clin Microbiol. 1998 Dec;36(12):3689-90. doi: 10.1128/JCM.36.12.3689-3690.1998.

Abstract

We have developed a PCR-based method to detect macrolide resistance and the virulence gene cagA in Helicobacter pylori within 24 h, thereby improving the lengthy process of culture-based approaches. Total DNA was prepared directly from stomach biopsy specimens. The procedure proved to be rapid and reliable and could be utilized for diagnostic purposes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial*
  • Bacterial Proteins / genetics*
  • Clarithromycin / pharmacology
  • Drug Resistance, Microbial / genetics
  • Genes, Bacterial*
  • Helicobacter pylori / drug effects
  • Helicobacter pylori / genetics*
  • Helicobacter pylori / pathogenicity
  • Humans
  • Polymerase Chain Reaction*
  • Stomach / microbiology*
  • Virulence

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • cagA protein, Helicobacter pylori
  • Clarithromycin