A comparative analysis of Ki-67, p53, and p21WAF1CIP1 expression in tamoxifen associated endometrial carcinomas

Anticancer Res. 1998 Nov-Dec;18(6B):4661-5.

Abstract

Objective: To determine clinicopathologic parameters, expression of proliferation markers, and immunohistochemical oncogene expression in endometrial cancers in patients with a history of breast cancer with and without tamoxifen use.

Methods: Thirty endometrial carcinoma specimens were examined from patients with a previous history of breast cancer. Patients who had taken tamoxifen (15) were compared to non-users (15). Immunohistochemical staining was performed for p53, Ki-67, and p21WAF1/CIP1, overexpression was defined as greater than 10% positivity.

Results: Patient populations were statistically similar. P53 was overexpressed in 73% of tamoxifen users compared to 53% of non-users. Ki-67 was overexpressed in over 90% of user and non-user specimens. p21WAF1/CIP1 was overexpressed in 33% of users and 47% of non-users. Tamoxifen users had shorter time to diagnosis of endometrial cancer than non-users.

Conclusions: In this small study, tamoxifen associated tumors expressed p53 more frequently than non-users, while the opposite was observed with p21WAF1/CIP1. This suggests that p53 mutations might play a role in development of tamoxifen associated tumors.

Publication types

  • Comparative Study

MeSH terms

  • Antineoplastic Agents, Hormonal / adverse effects*
  • Breast Neoplasms / drug therapy*
  • Cell Nucleus / pathology
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins / analysis*
  • Cyclins / genetics
  • Endometrial Neoplasms / chemically induced*
  • Endometrial Neoplasms / genetics
  • Endometrial Neoplasms / pathology*
  • Enzyme Inhibitors / analysis
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Ki-67 Antigen / analysis*
  • Ki-67 Antigen / genetics
  • Neoplasm Staging
  • Tamoxifen / adverse effects*
  • Tumor Suppressor Protein p53 / analysis*
  • Tumor Suppressor Protein p53 / genetics

Substances

  • Antineoplastic Agents, Hormonal
  • CDKN1A protein, human
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins
  • Enzyme Inhibitors
  • Ki-67 Antigen
  • Tumor Suppressor Protein p53
  • Tamoxifen