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Use of indirect site-directed mutagenesis to alter the substrate specificity of methylamine dehydrogenase.
Wang Y, Sun D, Davidson VL. Wang Y, et al. Among authors: sun d. J Biol Chem. 2002 Feb 8;277(6):4119-22. doi: 10.1074/jbc.M109270200. Epub 2001 Dec 3. J Biol Chem. 2002. PMID: 11733518 Free article.
When alphaPhe(55) is replaced with Ala, this preference is reversed with alphaF55A MADH preferring long-chain amines with at least seven carbons (Zhu, Z., Sun, D., and Davidson, V. L. (2000) Biochemistry 39, 11184-11186). To further modulate the substrate specificit …
When alphaPhe(55) is replaced with Ala, this preference is reversed with alphaF55A MADH preferring long-chain amines with at least seven car …
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