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Novel application of a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency in field studies.
Hanekom WA, Hughes J, Mavinkurve M, Mendillo M, Watkins M, Gamieldien H, Gelderbloem SJ, Sidibana M, Mansoor N, Davids V, Murray RA, Hawkridge A, Haslett PA, Ress S, Hussey GD, Kaplan G. Hanekom WA, et al. Among authors: sidibana m. J Immunol Methods. 2004 Aug;291(1-2):185-95. doi: 10.1016/j.jim.2004.06.010. J Immunol Methods. 2004. PMID: 15345316
Qualification of a whole blood intracellular cytokine staining assay to measure mycobacteria-specific CD4 and CD8 T cell immunity by flow cytometry.
Kagina BM, Mansoor N, Kpamegan EP, Penn-Nicholson A, Nemes E, Smit E, Gelderbloem S, Soares AP, Abel B, Keyser A, Sidibana M, Hughes JE, Kaplan G, Hussey GD, Hanekom WA, Scriba TJ. Kagina BM, et al. Among authors: sidibana m. J Immunol Methods. 2015 Feb;417:22-33. doi: 10.1016/j.jim.2014.12.003. Epub 2014 Dec 16. J Immunol Methods. 2015. PMID: 25523923 Free PMC article.
Specific T cell frequency and cytokine expression profile do not correlate with protection against tuberculosis after bacillus Calmette-Guérin vaccination of newborns.
Kagina BM, Abel B, Scriba TJ, Hughes EJ, Keyser A, Soares A, Gamieldien H, Sidibana M, Hatherill M, Gelderbloem S, Mahomed H, Hawkridge A, Hussey G, Kaplan G, Hanekom WA; other members of the South African Tuberculosis Vaccine Initiative. Kagina BM, et al. Among authors: sidibana m. Am J Respir Crit Care Med. 2010 Oct 15;182(8):1073-9. doi: 10.1164/rccm.201003-0334OC. Epub 2010 Jun 17. Am J Respir Crit Care Med. 2010. PMID: 20558627 Free PMC article.