Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates.
Sanchis J, Fernández L, Carballeira JD, Drone J, Gumulya Y, Höbenreich H, Kahakeaw D, Kille S, Lohmer R, Peyralans JJ, Podtetenieff J, Prasad S, Soni P, Taglieber A, Wu S, Zilly FE, Reetz MT.
Sanchis J, et al. Among authors: zilly fe.
Appl Microbiol Biotechnol. 2008 Nov;81(2):387-97. doi: 10.1007/s00253-008-1678-9. Epub 2008 Sep 27.
Appl Microbiol Biotechnol. 2008.
PMID: 18820909
Free PMC article.