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A cell-based reporter assay for screening for EcR agonist/antagonist activity of natural ecdysteroids in Lepidoptera (Bm5) and Diptera (S2) cell cultures, followed by modeling of ecdysteroid-EcR interactions and normal mode analysis.
Zotti MJ, De Geyter E, Swevers L, Braz AS, Scott LP, Rougé P, Coll J, Grutzmacher AD, Lenardão EJ, Smagghe G. Zotti MJ, et al. Among authors: swevers l. Pestic Biochem Physiol. 2013 Nov;107(3):309-20. doi: 10.1016/j.pestbp.2013.09.003. Epub 2013 Sep 26. Pestic Biochem Physiol. 2013. PMID: 24267692
Towards Coleoptera-specific high-throughput screening systems for compounds with ecdysone activity: development of EcR reporter assays using weevil (Anthonomus grandis)-derived cell lines and in silico analysis of ligand binding to A. grandis EcR ligand-binding pocket.
Soin T, Iga M, Swevers L, Rougé P, Janssen CR, Smagghe G. Soin T, et al. Among authors: swevers l. Insect Biochem Mol Biol. 2009 Aug;39(8):523-34. doi: 10.1016/j.ibmb.2009.06.003. Epub 2009 Jun 21. Insect Biochem Mol Biol. 2009. PMID: 19545627
121 results