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Engineering an allosteric transcription factor to respond to new ligands.
Taylor ND, Garruss AS, Moretti R, Chan S, Arbing MA, Cascio D, Rogers JK, Isaacs FJ, Kosuri S, Baker D, Fields S, Church GM, Raman S. Taylor ND, et al. Among authors: fields s. Nat Methods. 2016 Feb;13(2):177-83. doi: 10.1038/nmeth.3696. Epub 2015 Dec 21. Nat Methods. 2016. PMID: 26689263 Free PMC article.
Global mapping of protein-DNA interactions in vivo by digital genomic footprinting.
Hesselberth JR, Chen X, Zhang Z, Sabo PJ, Sandstrom R, Reynolds AP, Thurman RE, Neph S, Kuehn MS, Noble WS, Fields S, Stamatoyannopoulos JA. Hesselberth JR, et al. Among authors: fields s. Nat Methods. 2009 Apr;6(4):283-9. doi: 10.1038/nmeth.1313. Epub 2009 Mar 22. Nat Methods. 2009. PMID: 19305407 Free PMC article.
High-resolution mapping of protein sequence-function relationships.
Fowler DM, Araya CL, Fleishman SJ, Kellogg EH, Stephany JJ, Baker D, Fields S. Fowler DM, et al. Among authors: fields s. Nat Methods. 2010 Sep;7(9):741-6. doi: 10.1038/nmeth.1492. Epub 2010 Aug 15. Nat Methods. 2010. PMID: 20711194 Free PMC article.
Engineering allostery.
Raman S, Taylor N, Genuth N, Fields S, Church GM. Raman S, et al. Among authors: fields s. Trends Genet. 2014 Dec;30(12):521-8. doi: 10.1016/j.tig.2014.09.004. Epub 2014 Oct 8. Trends Genet. 2014. PMID: 25306102 Free PMC article. Review.
Massively parallel single-amino-acid mutagenesis.
Kitzman JO, Starita LM, Lo RS, Fields S, Shendure J. Kitzman JO, et al. Among authors: fields s. Nat Methods. 2015 Mar;12(3):203-6, 4 p following 206. doi: 10.1038/nmeth.3223. Epub 2015 Jan 5. Nat Methods. 2015. PMID: 25559584 Free PMC article.
A general strategy to construct small molecule biosensors in eukaryotes.
Feng J, Jester BW, Tinberg CE, Mandell DJ, Antunes MS, Chari R, Morey KJ, Rios X, Medford JI, Church GM, Fields S, Baker D. Feng J, et al. Among authors: fields s. Elife. 2015 Dec 29;4:e10606. doi: 10.7554/eLife.10606. Elife. 2015. PMID: 26714111 Free PMC article.
Engineered Biosensors from Dimeric Ligand-Binding Domains.
Jester BW, Tinberg CE, Rich MS, Baker D, Fields S. Jester BW, et al. Among authors: fields s. ACS Synth Biol. 2018 Oct 19;7(10):2457-2467. doi: 10.1021/acssynbio.8b00242. Epub 2018 Sep 25. ACS Synth Biol. 2018. PMID: 30204430 Free PMC article.
760 results