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A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid.
Tálas A, Kulcsár PI, Weinhardt N, Borsy A, Tóth E, Szebényi K, Krausz SL, Huszár K, Vida I, Sturm Á, Gordos B, Hoffmann OI, Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E. Tálas A, et al. Among authors: welker e. DNA Res. 2017 Dec 1;24(6):609-621. doi: 10.1093/dnares/dsx029. DNA Res. 2017. PMID: 28679166 Free PMC article.
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Tóth E, Czene BC, Kulcsár PI, Krausz SL, Tálas A, Nyeste A, Varga É, Huszár K, Weinhardt N, Ligeti Z, Borsy AÉ, Fodor E, Welker E. Tóth E, et al. Among authors: welker e. Nucleic Acids Res. 2018 Nov 2;46(19):10272-10285. doi: 10.1093/nar/gky815. Nucleic Acids Res. 2018. PMID: 30239882 Free PMC article.
144 results