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DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP.
Bruce EA, Huang ML, Perchetti GA, Tighe S, Laaguiby P, Hoffman JJ, Gerrard DL, Nalla AK, Wei Y, Greninger AL, Diehl SA, Shirley DJ, Leonard DGB, Huston CD, Kirkpatrick BD, Dragon JA, Crothers JW, Jerome KR, Botten JW. Bruce EA, et al. Among authors: crothers jw. bioRxiv [Preprint]. 2020 Apr 6:2020.03.20.001008. doi: 10.1101/2020.03.20.001008. bioRxiv. 2020. Update in: PLoS Biol. 2020 Oct 2;18(10):e3000896. doi: 10.1371/journal.pbio.3000896 PMID: 32511328 Free PMC article. Updated. Preprint.
Kinetics and Isotype Assessment of Antibodies Targeting the Spike Protein Receptor Binding Domain of SARS-CoV-2 In COVID-19 Patients as a function of Age and Biological Sex.
Graham NR, Whitaker AN, Strother CA, Miles AK, Grier D, McElvany BD, Bruce EA, Poynter ME, Pierce KK, Kirkpatrick BD, Stapleton RD, An G, Botten JW, Crothers JW, Diehl SA. Graham NR, et al. Among authors: crothers jw. medRxiv [Preprint]. 2020 Jul 16:2020.07.15.20154443. doi: 10.1101/2020.07.15.20154443. medRxiv. 2020. Update in: Clin Transl Immunology. 2020 Oct 07;9(10):e1189. doi: 10.1002/cti2.1189 PMID: 32743592 Free PMC article. Updated. Preprint.
Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step.
Bruce EA, Huang ML, Perchetti GA, Tighe S, Laaguiby P, Hoffman JJ, Gerrard DL, Nalla AK, Wei Y, Greninger AL, Diehl SA, Shirley DJ, Leonard DGB, Huston CD, Kirkpatrick BD, Dragon JA, Crothers JW, Jerome KR, Botten JW. Bruce EA, et al. Among authors: crothers jw. PLoS Biol. 2020 Oct 2;18(10):e3000896. doi: 10.1371/journal.pbio.3000896. eCollection 2020 Oct. PLoS Biol. 2020. PMID: 33006983 Free PMC article.
Kinetics and isotype assessment of antibodies targeting the spike protein receptor-binding domain of severe acute respiratory syndrome-coronavirus-2 in COVID-19 patients as a function of age, biological sex and disease severity.
Graham NR, Whitaker AN, Strother CA, Miles AK, Grier D, McElvany BD, Bruce EA, Poynter ME, Pierce KK, Kirkpatrick BD, Stapleton RD, An G, van den Broek-Altenburg E, Botten JW, Crothers JW, Diehl SA. Graham NR, et al. Among authors: crothers jw. Clin Transl Immunology. 2020 Oct 7;9(10):e1189. doi: 10.1002/cti2.1189. eCollection 2020. Clin Transl Immunology. 2020. PMID: 33072323 Free PMC article.
An international, interlaboratory ring trial confirms the feasibility of an extraction-less "direct" RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples.
Mills MG, Bruce E, Huang ML, Crothers JW, Hyrien O, Oura CAL, Blake L, Brown Jordan A, Hester S, Wehmas L, Mari B, Barby P, Lacoux C, Fassy J, Vial P, Vial C, Martinez JRW, Oladipo OO, Inuwa B, Shittu I, Meseko CA, Chammas R, Santos CF, Dionísio TJ, Garbieri TF, Parisi VA, Mendes-Correa MC, de Paula AV, Romano CM, Góes LGB, Minoprio P, Campos AC, Cunha MP, Vilela APP, Nyirenda T, Mkakosya RS, Muula AS, Dumm RE, Harris RM, Mitchell CA, Pettit S, Botten J, Jerome KR. Mills MG, et al. Among authors: crothers jw. PLoS One. 2022 Jan 13;17(1):e0261853. doi: 10.1371/journal.pone.0261853. eCollection 2022. PLoS One. 2022. PMID: 35025926 Free PMC article.
Intradermal fractional-dose inactivated polio vaccine (fIPV) adjuvanted with double mutant Enterotoxigenic Escherichia coli heat labile toxin (dmLT) is well-tolerated and augments a systemic immune response to all three poliovirus serotypes in a randomized placebo-controlled trial.
Crothers JW, Ross Colgate E, Cowan KJ, Dickson DM, Walsh M, Carmolli M, Wright PF, Norton EB, Kirkpatrick BD. Crothers JW, et al. Vaccine. 2022 Apr 26;40(19):2705-2713. doi: 10.1016/j.vaccine.2022.03.056. Epub 2022 Mar 30. Vaccine. 2022. PMID: 35367069 Free PMC article. Clinical Trial.
Corrigendum to "Intradermal fractional-dose inactivated polio vaccine (fIPV) adjuvanted with double mutant Enterotoxigenic Escherichia coli heat labile toxin (dmLT) is well-tolerated and augments a systemic immune response to all three poliovirus serotypes in a randomized active-controlled trial" [Vaccine 40(19) (2022) 2705-2713].
Crothers JW, Ross Colgate E, Cowan KJ, Dickson DM, Walsh M, Carmolli M, Wright PF, Norton EB, Kirkpatrick BD. Crothers JW, et al. Vaccine. 2022 May 31;40(25):3481. doi: 10.1016/j.vaccine.2022.05.001. Epub 2022 May 6. Vaccine. 2022. PMID: 35534312 Free PMC article. No abstract available.
Viral infectivity in paediatric SARS-CoV-2 clinical samples does not vary by age.
Schmidt MM, Despres HW, Shirley DJ, Bose ME, McCaul KC, Crothers JW, Henrickson KJ, Lee B, Bruce EA. Schmidt MM, et al. Among authors: crothers jw. Access Microbiol. 2023 May 26;5(5):acmi000547.v4. doi: 10.1099/acmi.0.000547.v4. eCollection 2023. Access Microbiol. 2023. PMID: 37323941 Free PMC article.
Determining the impact of vaccination on SARS-CoV-2 RT-PCR cycle threshold values and infectious viral titres.
Peterson KL, Snyder JP, Despres HW, Schmidt MM, Eckstrom KM, Unger AL, Carmolli MP, Sevigny JL, Shirley DJ, Dragon JA, Thomas WK, Bruce EA, Crothers JW. Peterson KL, et al. Among authors: crothers jw. Access Microbiol. 2023 Oct 20;5(10):000597.v3. doi: 10.1099/acmi.0.000597.v3. eCollection 2023. Access Microbiol. 2023. PMID: 37970082 Free PMC article.
33 results