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An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography.
Butryn A, Simon PS, Aller P, Hinchliffe P, Massad RN, Leen G, Tooke CL, Bogacz I, Kim IS, Bhowmick A, Brewster AS, Devenish NE, Brem J, Kamps JJAG, Lang PA, Rabe P, Axford D, Beale JH, Davy B, Ebrahim A, Orlans J, Storm SLS, Zhou T, Owada S, Tanaka R, Tono K, Evans G, Owen RL, Houle FA, Sauter NK, Schofield CJ, Spencer J, Yachandra VK, Yano J, Kern JF, Orville AM. Butryn A, et al. Among authors: massad rn. Nat Commun. 2021 Jul 22;12(1):4461. doi: 10.1038/s41467-021-24757-7. Nat Commun. 2021. PMID: 34294694 Free PMC article.
Effects of x-ray free-electron laser pulse intensity on the Mn K β 1,3 x-ray emission spectrum in photosystem II-A case study for metalloprotein crystals and solutions.
Fransson T, Alonso-Mori R, Chatterjee R, Cheah MH, Ibrahim M, Hussein R, Zhang M, Fuller F, Gul S, Kim IS, Simon PS, Bogacz I, Makita H, de Lichtenberg C, Song S, Batyuk A, Sokaras D, Massad R, Doyle M, Britz A, Weninger C, Zouni A, Messinger J, Yachandra VK, Yano J, Kern J, Bergmann U. Fransson T, et al. Struct Dyn. 2021 Nov 22;8(6):064302. doi: 10.1063/4.0000130. eCollection 2021 Nov. Struct Dyn. 2021. PMID: 34849380 Free PMC article.
XFEL serial crystallography reveals the room temperature structure of methyl-coenzyme M reductase.
Ohmer CJ, Dasgupta M, Patwardhan A, Bogacz I, Kaminsky C, Doyle MD, Chen PY, Keable SM, Makita H, Simon PS, Massad R, Fransson T, Chatterjee R, Bhowmick A, Paley DW, Moriarty NW, Brewster AS, Gee LB, Alonso-Mori R, Moss F, Fuller FD, Batyuk A, Sauter NK, Bergmann U, Drennan CL, Yachandra VK, Yano J, Kern JF, Ragsdale SW. Ohmer CJ, et al. J Inorg Biochem. 2022 May;230:111768. doi: 10.1016/j.jinorgbio.2022.111768. Epub 2022 Feb 17. J Inorg Biochem. 2022. PMID: 35202981 Free PMC article.