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Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acids.
Nguyen LT, Rananaware SR, Yang LG, Macaluso NC, Ocana-Ortiz JE, Meister KS, Pizzano BLM, Sandoval LSW, Hautamaki RC, Fang ZR, Joseph SM, Shoemaker GM, Carman DR, Chang L, Rakestraw NR, Zachary JF, Guerra S, Perez A, Jain PK. Nguyen LT, et al. Among authors: hautamaki rc. Cell Rep Med. 2023 May 16;4(5):101037. doi: 10.1016/j.xcrm.2023.101037. Epub 2023 May 8. Cell Rep Med. 2023. PMID: 37160120 Free PMC article.
Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs.
Nguyen LT, Macaluso NC, Rakestraw NR, Carman DR, Pizzano BLM, Hautamaki RC, Rananaware SR, Roberts IE, Jain PK. Nguyen LT, et al. Among authors: hautamaki rc. Cell Rep. 2024 Feb 27;43(2):113777. doi: 10.1016/j.celrep.2024.113777. Epub 2024 Feb 14. Cell Rep. 2024. PMID: 38358883 Free PMC article.