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Regulation of K+ flow by a ring of negative charges in the outer pore of BKCa channels. Part I: Aspartate 292 modulates K+ conduction by external surface charge effect.
Haug T, Sigg D, Ciani S, Toro L, Stefani E, Olcese R. Haug T, et al. Among authors: stefani e. J Gen Physiol. 2004 Aug;124(2):173-84. doi: 10.1085/jgp.200308949. J Gen Physiol. 2004. PMID: 15277578 Free PMC article.
Exchanging the aspartate residue with asparagine in this sequence abolishes ionic conductance of the Shaker K+ channel (D447N) (Hurst, R.S., L. Toro, and E. Stefani. 1996. FEBS Lett. 388:59-65). In contrast, we found that the corresponding mutation (D292N) in the po …
Exchanging the aspartate residue with asparagine in this sequence abolishes ionic conductance of the Shaker K+ channel (D447N) (Hurst, R.S., …
Regulation of K+ flow by a ring of negative charges in the outer pore of BKCa channels. Part II: Neutralization of aspartate 292 reduces long channel openings and gating current slow component.
Haug T, Olcese R, Toro L, Stefani E. Haug T, et al. Among authors: stefani e. J Gen Physiol. 2004 Aug;124(2):185-97. doi: 10.1085/jgp.200308950. J Gen Physiol. 2004. PMID: 15277579 Free PMC article.
Neutralization of the aspartate near the selectivity filter in the GYGD pore sequence (D292N) of the voltage- and Ca(2+)-activated K+ channel (MaxiK, BKCa) does not prevent conduction like the corresponding mutation in Shaker channel, but profoundly affects major biophysical prop …
Neutralization of the aspartate near the selectivity filter in the GYGD pore sequence (D292N) of the voltage- and Ca(2+)-activated K+ channe …
Intracellular BK(Ca) (iBK(Ca)) channels.
Singh H, Stefani E, Toro L. Singh H, et al. Among authors: stefani e. J Physiol. 2012 Dec 1;590(23):5937-47. doi: 10.1113/jphysiol.2011.215533. Epub 2012 Aug 28. J Physiol. 2012. PMID: 22930268 Free PMC article. Review.
Identification of a second region of the beta-subunit involved in regulation of calcium channel inactivation.
Qin N, Olcese R, Zhou J, Cabello OA, Birnbaumer L, Stefani E. Qin N, et al. Among authors: stefani e. Am J Physiol. 1996 Nov;271(5 Pt 1):C1539-45. doi: 10.1152/ajpcell.1996.271.5.C1539. Am J Physiol. 1996. PMID: 8944637
Olcese, N. Qin, T. Schneider, A. Neely, X. Wei, E. Stefani, and L. Birnbaumer, Neuron 13: 1433-1438, 1994). By constructing NH2-terminal deletions of several splice variants of beta-subunits, we have now found differences in the way they affect the rate of alpha 1E …
Olcese, N. Qin, T. Schneider, A. Neely, X. Wei, E. Stefani, and L. Birnbaumer, Neuron 13: 1433-1438, 1994). By constructing NH …
596 results