Heterologous high level expression, purification, and enzymological properties of recombinant rat cobalamin-dependent methionine synthase.
Yamada K, Yamada S, Tobimatsu T, Toraya T.
Yamada K, et al. Among authors: yamada s.
J Biol Chem. 1999 Dec 10;274(50):35571-6. doi: 10.1074/jbc.274.50.35571.
J Biol Chem. 1999.
PMID: 10585432
Free article.
Rat methionine synthase was expressed chiefly as apoenzyme in recombinant baculovirus-infected insect cells (Yamada, K., Tobimatsu, T., and Toraya, T. (1998) Biosci. Biotech. Biochem. 62, 2155-2160). The apoenzyme produced was very unstable, and therefore, after com …
Rat methionine synthase was expressed chiefly as apoenzyme in recombinant baculovirus-infected insect cells (Yamada, K., Tobim …